Journal: Applied and Environmental Microbiology

Article Title: Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

doi: 10.1128/aem.00870-16

Figure Lengend Snippet: Figure 4 shows XDLVO energy profiles of virion-virion inter- facial interactions at different pH values. For all pH values except 7.6, the calculations were performed for HAdV in 1 mM NaCl. For pH 7.6, size and -potential values used in XDLVO calculations were for virions in the recommended storage buffer (1 mM EDTA plus 10 mM Tris). For pH values of 5.8, the height of the energy barrier increases with pH, with the sole exception of pH 7.6. The deviation from the trend is due to the different background solu- tion (10 mM Tris plus 1 mM EDTA instead of 1 mM NaCl) (see “Size, -potential, and surface charge density of HAdV 40”). The negligible aggregation of virions for pH values of 5.8 is due to the high energy barrier (24.5 kT to 52.6 kT). At pH 4.7, where the energy barrier is 8.0 kT, significant aggregation occurs. At pH 4.0 and pH 4.3, the energy barrier decreases to below 1.2 kT, leading to further aggregation. Due to the high positive -potential (29.4 mV) at pH 2.8, the energy barrier is 20.7 kT and the virion sus- pension is stable. XDLVO energies of virion-membrane interfacial interac- tions in membrane feed solutions made with DI water and tap water. XDLVO calculations for virion-membrane interactions in DI water were performed for an ionic strength of 0.2 mM; this is the approximate ionic strength determined from conductivity measurements of DI water spiked with HAdV 40 stock. However, the streaming potential of the membrane could be measured only with 1 mM NaCl electrolytes, so this value was used to approxi- mate the membrane charge in the HAdV-spiked DI water. The approximation is reasonable because the electrophoretic mobili- ties of virions in DI water (1.65 0.19 m · S1 · V1 · cm at pH 5.8) and in 1 mM NaCl solution (1.72 0.48 m · S1 · V1 · cm at pH 5.8) were not statistically different.

Article Snippet: All samples were characterized in terms of pH (Orion 3-Star Plus meter; Thermo Electron Corp., Waltham, MA), conductivity (Orion 550 conductivity meter; Thermo Electron Corp., Waltham, MA), total organic carbon (TOC) content, and concentrations of key cations.

Techniques: Membrane

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: Expression of SLC12A7 in gastric cancer cell lines before and after siRNA knockdown (A–C) mRNA expression levels and protein expression levels of SLC12A7 were elevated in all 2 GC cell lines HGC27, MGC803 compared with normal stomach epithelial cell line GES-1 (D) mRNA expression levels and protein expression levels of SLC12A7 in HGC27 cells (E) mRNA expression levels and protein expression levels of SLC12A7 in MGC803 cells. All assays were performed in triplicate (ns represents not significant; * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001; **** represents p < 0.0001).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Expressing, Knockdown

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: Knockdown of SLC12A7 inhibited proliferation, metastasis, and EMT process in GC cell lines (A,B) Knockdown of SLC12A7 led to cancer proliferation inhibition in HGC27 and MGC803 cell lines (C,D) Knockdown of SLC12A7 reduced the migration and invasion of GC cells. (E,F) Knockdown of SLC12A7 exhibited prominent E-Cadherin upregulation and Vimentin downregulation. All assays were performed in triplicate (* represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Knockdown, Inhibition, Migration

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: Knockdown of SLC12A7 led to cell cycle arrest (A,B) CDK1 and Cyclin A2 were downregulated in HGC27 and MGC803 cells after SLC12A7 inhibition (C,D) Flow cytometry results indicated an increase in the percentage of G2-phase cells and a decrease in the percentage of S-phase cells after SLC12A7 knockdown, suggesting G2/M cell-cycle arrest. All assays were performed in triplicate (* represents p < 0.05; ** represents p < 0.01).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Knockdown, Inhibition, Flow Cytometry

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: Knockdown of SLC12A7 resulted in apoptosis in GC cells (A,C) Flow cytometry results showed elevated apoptosis rates in HGC27 and MGC803 cells after application of siRNA inhibition (B) Expression levels of apoptosis-related protein in HGC27 and MGC803 cells after SLC12A7 inhibition. All assays were performed in triplicate (ns represents not significant; * represents p < 0.05; *** represents p < 0.001; **** represents p < 0.0001).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Knockdown, Flow Cytometry, Inhibition, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: Knockdown of SLC12A7 influenced PI3K/AKT signaling pathway in GC cells (A,B) Western blot results of PI3K and AKT total protein expression levels and phosphorylated protein expression levels in HGC27 and MGC803 cells after knocking down SLC12A7 . All assays were performed in triplicate (ns represents not significant; * represents p < 0.05; ** represents p < 0.01).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Knockdown, Western Blot, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC12A7 promotes malignant phenotypes of gastric cancer cells and is associated with Ezrin-related PI3K/AKT signaling

doi: 10.3389/fcell.2026.1828288

Figure Lengend Snippet: SLC12A7 knockdown attenuates PI3K/AKT phosphorylation and Ezrin expression in gastric cancer cells (A) Correlation analysis between SLC12A7 and Ezrin (B–D) Western blot analysis of PI3K, p-PI3K, AKT, p-AKT, and Ezrin protein expression levels in HGC27 and MGC803 cells after co-transfected with si- SLC12A7 and Ezrin (E) Docking simulation of SLC12A7 and Ezrin molecules. All assays were performed in triplicate (ns represents not significant; * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001) (F) SLC12A7 and Ezrin show immunofluorescent colocalization in MGC803 (PCC = 0.80) and HGC27 (PCC = 0.69).

Article Snippet: For cell apoptosis rate detection, HGC27 and MGC803 cells were pretreated separately using si- SLC12A7 or si-NC for 48 h. A total of 1 × 10 6 cells at the logarithmic phase of growth were harvested according to manufacturer’s protocol: rinsed twice with PBS, each sample resuspended using a 100 μL 1×binding buffer, 5 μL of FITC-Annexin V and 5 μL of propidium iodide (PI) (MK1028, Boster, Wuhan, China) added and incubated on ice in the dark for 15 min. Another 400 μL of 1×binding buffer was added to resuspend the cells.

Techniques: Knockdown, Phospho-proteomics, Expressing, Analysis, Western Blot, Transfection, Docking Assay